Magnetic Cell Isolation with Fab-TACS® and MHC I Streptamer®
Fab-TACS® and MHC I Streptamer®: fully reversible reagents for the selection of uncompromised, authentic cells
In order to study cell-cell interactions, it is essential to gain unmanipulated cells from cell isolation. The Fab-TACS® and Streptamer® technologies cover different stages in cell research from cell isolation to cell expansion and cell staining. With this method the cells preserve authentic properties, full effector function as well as viability and serial positive selections of even rare cell subsets is possible. By adding low concentrations of biotin, the isolation reagents dissociate rapidly from the cell surface, leaving authentic cells ready for further experiments.
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Fab-TACS® and MHC I Streptamer® - Key features
- No stimulation of cells after cell separation
- Optimal preservation of cell effector function
- Improved viability of cells
- High reproducibility
Full reversibility in detail
Low affinity Strep-tagged Fab fragments or MHC molecules are employed to convey the specificity to the target cell population. In order to achieve a high binding affinity for proper cell isolation, the Strep-tag® fragments bind to a multimerized Strep-Tactin®, a streptavidin derivative. Due to this a high binding avidity of the fragment is achieved. Upon addition of low concentrations of biotin, isolation reagents dissociate rapidly from the cell surface. This reversibility enables sequential positive cell selections with magnetic beads.
A modular system for cell selection AND staining
Fab-Streps or MHC-Streps can be combined with bead-labeled Strep-Tactin® for magnetic isolation. Biotin is used for removing the cell isolation reagents. This modular system enables the combination of multiple surface marker- or antigen-specific staining and isolation products, making your experiments more convenient and economic. All reagents can be purchased as single products or assembled in ready-to-use staining or isolation kits.