5-5222-001

pDSG-IBA105 vector

Mammalian expression vector encoding an N-terminal Twin-Strep-tag®

€219.00*

Content
pDSG-IBA105 is a small transient expression vector encoding an N-terminal Twin-Strep-tag® and was especially developed for the use in combination with the MEXi mammalian expression system. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and the ColE1 replication origin (pUC) for a high plasmid copy number. In addition, it contains the human cytomegalovirus (CMV) immediate-early promoter for high-level expression and the origin of replication from Epstein-Barr Virus (oriP) for extrachromosomal replication driven by EBNA-1 expressed by MEXi-293E cells. Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

Affinity Tag N'-terminal: Twin-Strep-tag®
Cloning Method: Direct cloning using restriction enzyme LguI
Concentration: 250 ng/µl
Expression Host: Mammalian cells, MEXi-293E cells
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in mammalian cells
Promoter: CMV Promoter
Resistance: Ampicillin
Sequence: See Documents
Size: 5819 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Competent E. coli TOP10
Chemically competent cells for plasmid propagation
MEXi-293E cells
HEK293 cell line for transient expression of recombinant proteins
MEXi-CM
Culture medium for MEXi-293E cells
MEXi-TM
Transfection medium for MEXi-293E cells
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

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