2-6001-001

MEXi-293E cells

HEK293 cell line for transient expression of recombinant proteins

€356.00*

Content

MEXi-293E cells are human embryonic kidney (HEK) cells derived from the 293 cell line and used for propagation of plasmids and expression of recombinant proteins in mammalian cells. The cell line expresses the EBNA-1 protein for episomal replication of oriP-harboring plasmids (pDSG-IBA vectors), is adapted for growth in suspension, and for use with the MEXi cell culture medium. The cells are part of the MEXi mammalian expression system.

The MEXi system was developed to offer an affordable mammalian protein expression system coming along with all essential components: 
• MEXi-293E cell line 
• A variety of pDSG expression vectors
• MEXi-CM culture medium
• MEXi-TM transfection medium 

Further advantages:
• Transient expression for time- and cost-efficient protein production
• Multiple expression vectors with different features
• Optimized for Twin-Strep-tag®:Strep-Tactin®XT system for highly pure proteins
• IP-free system

Specifications

Biosafety Level: S1 (Germany), BSL-2 (USA)
Form: Cryopreserved
Growth Properties: Suspension culture
Possible Application: Expression host for recombinat Strep-tag®II and Twin-Strep-tag® proteins

Shipping information

Storage: -140 to -196 °C
Stability: 6 months after shipping
Shipping: Dry ice

Accessories

MEXi-CM
Culture medium for MEXi-293E cells
MEXi-TM
Transfection medium for MEXi-293E cells
Avidin
Efficient blocking of biotin and biotinlyated proteins
BioLock
Biotin blocking solution for lysates and culture supernatants
pDSG-IBAwt1 vector
Mammalian expression vector without affinity tag
pDSG-IBAwt2 vector
Mammalian expression vector for secreted proteins without affinity tag
pDSG-IBA43 vector
Mammalian expression vector encoding an N-terminal His-tag and C-terminal Strep-tag®II
pDSG-IBA45 vector
Mammalian expression vector encoding an N-terminal Strep-tag®II and C-terminal His-tag
pDSG-IBA102 vector
Mammalian expression vector for secretion of C-terminal Twin-Strep-tag® fusion proteins
pDSG-IBA103 vector
Mammalian expression vector encoding a C-terminal Twin-Strep-tag®
pDSG-IBA104 vector
Mammalian expression vector for secretion of N-terminal Twin-Strep-tag® fusion proteins
pDSG-IBA105 vector
Mammalian expression vector encoding an N-terminal Twin-Strep-tag®

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Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® cartridge
HPLC/FPLC cartridge to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity cartridge
HPLC/FPLC cartridge to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
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