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pDSG-IBAwt2 vector

Mammalian expression vector for secreted proteins without affinity tag


Content: 1 Stk
pDSG-IBAwt2 is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and the ColE1 replication origin (pUC) for a high plasmid copy number. In addition, it contains the human cytomegalovirus (CMV) immediate-early promoter for high-level expression and the origin of replication from Epstein-Barr Virus (oriP) for extrachromosomal replication driven by EBNA-1 expressed by MEXi-293E cells. The expressed protein is transferred into the medium due to the BM40 secretory signal peptide. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. Please note that cloning into pDSG-IBA acceptor vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called entry vector is possible.


Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: Mammalian cells, MEXi-293E cells
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in mammalian cells
Promoter: CMV Promoter
Resistance: Ampicillin
Sequence: See Documents
Size: 5771 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature


Competent E. coli TOP10
Chemically competent cells for plasmid propagation
MEXi-293E cells
HEK293 cell line for transient expression of recombinant proteins
Culture medium for MEXi-293E cells
Transfection medium for MEXi-293E cells
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

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