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BioLock
Biotin blocking solution for lysates and culture supernatants
BioLock is a ready-to-use biotin blocking solution containing avidin. Avidin strongly binds to biotin and biotinylated proteins, but not to Strep-tag®II or Twin-Strep-tag®-fusion proteins. This way, unspecific binding of biotin/biotinylated proteins to Strep-Tactin® or Strep-Tactin®XT resins is prevented, ensuring an unimpaired binding capacity and a high purity of the target protein.
Biotin and/or biotinylated proteins are present in cell lysates and cell culture media, especially from mammalian or insect cells. Thus, if Twin-Strep-tag® and Strep-tag®II fusion proteins from biotin-containing extracts or media are intended to be purified, biotin has to be masked by addition of BioLock to the sample prior to applying it to the resin.
For masking biotin for western blot, we recommend using purified avidin.
| Form: | Suspension |
|---|---|
| Possible Application: | Blocking of biotin in e.g. culture media |
| Size: | 17 kDa per subunit |
| Source: | Egg white |
| Specific Activity: | > 70 U/ml |
| Storage: | Room temperature |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Strep-Tactin® 4Flow® high capacity FPLC column
Replaces Strep-Tactin® Superflow® high capacity FPLC column
Strep-Tactin® 4Flow® high capacity resin
Replaces Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity
Strep-Tactin® 4Flow® high capacity column
Replaces Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity gravity flow columns
