2-1253-001

Strep-Tactin® 4Flow® high capacity column

Name: Strep-Tactin® 4Flow® high capacity column
Brand: IBA Lifesciences
SKU: 2-1253-001
Price: 369.0 EUR
Availability: InStock

Replaces Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity gravity flow columns

Content

5 x 0.2 ml

1 ml

5 ml

Strep-Tactin® 4Flow® high capacity columns replace Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity columns, providing identical performance and unifying the resins. Further information about the differences between Sepharose®, Superflow®, and 4Flow®, as well as the advantages of Strep-Tactin® 4Flow® high capacity, can be found here.

Strep-Tactin® 4Flow® high capacity columns are designed for purifying Strep-tag®II and Twin-Strep-tag® proteins via affinity chromatography in gravity flow. This product features a 4% cross-linked agarose beads coupled with Strep-Tactin®, a variant of streptavidin. Strep-Tactin® selectively binds to proteins fused with a Strep-tag®II or Twin-Strep-tag® and enables targeted elution via desthiobiotin. It can be regenerated with 100 mM NaOH for multiple uses, offering a cost-effective solution. This product serves as an alternative to our Strep-Tactin®XT 4Flow® resins for proteins that are challenging to elute. Please note that biotinylated proteins also bind to Strep-Tactin® 4Flow® high capacity. For samples containing biotinylated proteins, consider using BioLock. For comprehensive details on our available resins, including recommendations, refer to our resin specifications.

Specifications

Bead Size:	50 - 150 µm spherical
Form:	Pre-packed gravity flow column
Possible Application:	Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Protein Binding Capacity:	20 mg/ml resin
Specificity:	Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support:	4% agarose, crosslinked