Strep-Tactin®XT 4Flow® high capacity FPLC column
Strep-Tactin®XT 4Flow® high capacity FPLC columns contain 4% agarose resin, coupled to Strep‑Tactin®XT. The columns can be used for fully automated purification of Strep-tag®II or Twin-Strep-tag® fusion proteins with HPLC/FPLC devices, like Äkta®. The resin is suitable for all protein classes, especially low abundant or challenging proteins. The low concentration of agarose is suitable for the purification of targets with high molecular weights. In comparison to Strep-Tactin® 4Flow® resin, this high capacity variant has higher density of Strep-Tactin®XT, resulting in twice as much purified protein. The resin has broad pH compatibility, excellent pressure stability, strong binding affinity and delivers high target purity (> 95 %).
For the purification of multimeric proteins, we recommend using Strep-Tactin® Superflow® high capacity FPLC columns instead, since the increased avidity might influence elution efficiency.
Please note that this product was previously called Strep-Tactin®XT 4Flow® high capacity cartridge.
|Bead Size:||50 - 150 µm spherical|
|Dynamic Protein Binding Capacity:||14 mg/ml resin|
|Possible Application:||Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins|
|Specificity:||Twin-Strep-tag® and Strep-tag®II|
|Support:||4% agarose, crosslinked|
|Stability:||6 months after shipping|