Efficient purification of large proteins

High affinity meets high capacity

Efficient purification of large proteins can be challenging. If the agarose has a high concentration, the more difficult it is for large proteins to enter the agarose. As a result, large target proteins are insufficiently immobilized and remain in the flow-through. The solution to this problem is highly specific Strep-Tactin®XT combined with a low concentrated agarose, Strep-Tactin®XT 4Flow®.

Strep-Tactin®XT 4Flow® is the resin to start with due to particular applicability even for low abundant or challenging proteins. In addition, a further increase in protein yield can be achieved through the high capacity variant of Strep-Tactin®XT 4Flow®.

Special Offer

Save 25%* on 4Flow® products!

Type the promotion code  TEST4FLOW in your next order (email, online).

*This discount is valid until 19th July 2020 for one order per customer. Not valid in combination with other IBA discounts. Distributores and IBA staff excluded. Discount is valid for 4Flow® products only.

Key benefits:

  • Higher affinity and protein yield even for large proteins
  • Higher capacity for large proteins
  • Broad pH stability (pH 4-10)
  • Higher pressure stability compared to Strep-Tactin® Sepharose
  • Cost efficient
Comparison of the yield of a large human glycoprotein (129 kDa) purified with Strep-Tactin®XT Superflow® high capacity or Strep-Tactin®XT 4Flow® high capacity.
Comparison of the yield of a large human glycoprotein (129 kDa) purified with Strep-Tactin®XT Superflow® high capacity or Strep-Tactin®XT 4Flow® high capacity. With Strep-Tactin®XT 4Flow® high capacity, a 5-fold higher yield can be achieved.

All products at a glance

Format Size Cat. no.
classic capacity
Cat. no.
high capacity
Strep-Tactin®XT 4Flow® 50% suspension
4 ml
20 ml
50 ml
2-5010-002
2-5010-010
2-5010-025
2-5030-002
2-5030-010
2-5030-025
Strep-Tactin®XT 4Flow® gravity flow columns
5x 0.2 ml
1 ml
5 ml
10 ml
2-5011-005
2-5012-001
2-5013-001
2-5014-001
2-5031-005
2-5032-001
2-5033-001
2-5034-001
Strep-Tactin®XT 4Flow® cartridges
1 ml
5 ml
2-5021-001
2-5022-001
2-5025-001
2-5026-001
Strep-Tactin®XT 4Flow® Starter Kit 1 kit 2-5998-000
2-5998-000
Strep-Tactin®XT Regeneration Buffer, Buffer XT-R 250 ml 2-1045-250
2-1045-250