Efficient purification of large proteins

High affinity meets high capacity

Efficient purification of large proteins can be challenging. If the agarose has a high concentration, the more difficult it is for large proteins to enter the agarose. As a result, large target proteins are insufficiently immobilized and remain in the flow-through. The solution to this problem is highly specific Strep-Tactin®XT combined with a low concentrated agarose, Strep-Tactin®XT 4Flow®.

Strep-Tactin®XT 4Flow® is the resin to start with due to particular applicability even for low abundant or challenging proteins. In addition, a further increase in protein yield can be achieved through the high capacity variant of Strep-Tactin®XT 4Flow®.

Key benefits:

  • Higher affinity and protein yield even for large proteins
  • Higher capacity for large proteins
  • Broad pH stability (pH 4-10)
  • Higher pressure stability compared to Strep-Tactin® Sepharose
  • Cost efficient
Comparison of the yield of a large human glycoprotein (129 kDa) purified with Strep-Tactin®XT Superflow® high capacity or Strep-Tactin®XT 4Flow® high capacity.
Comparison of the yield of a large human glycoprotein (129 kDa) purified with Strep-Tactin®XT Superflow® high capacity or Strep-Tactin®XT 4Flow® high capacity. With Strep-Tactin®XT 4Flow® high capacity, a 5-fold higher yield can be achieved.

All products at a glance

Format Size Cat. no.
classic capacity
Cat. no.
high capacity
Strep-Tactin®XT 4Flow® 50% suspension
4 ml
20 ml
50 ml
2-5010-002
2-5010-010
2-5010-025
2-5030-002
2-5030-010
2-5030-025
Strep-Tactin®XT 4Flow® gravity flow columns
5x 0.2 ml
1 ml
5 ml
10 ml
2-5011-005
2-5012-001
2-5013-001
2-5014-001
2-5031-005
2-5032-001
2-5033-001
2-5034-001
Strep-Tactin®XT 4Flow® cartridges
1 ml
5 ml
2-5021-001
2-5022-001
2-5025-001
2-5026-001
Strep-Tactin®XT 4Flow® Starter Kit 1 kit 2-5998-000
2-5998-000
Strep-Tactin®XT Regeneration Buffer, Buffer XT-R 250 ml 2-1045-250
2-1045-250