Application examples
New applications of the 3rd generation Strep-tag® system
For batch purification IBA provides Strep-Tactin®XT coated magnetic beads: MagStrep "type3" XT beads. MagStrep "type3" XT beads allow the batch purification of highly pure proteins in Strep-tag® quality and the possibility of different elution procedures:
- Native conditions using biotin
- Denaturing conditions using sample buffer and boiling for elution
For quick and dirty test purification in batch format you can also use Strep-Tactin®XT Superflow® resin.
Example purification
Purification of GFP-Strep-tag fusion protein from crude bact. extract using MagStrep “type3” XT beads
The 3rd generation Strep-tag® system can now be used for purification under denaturing conditions using up to 6 M urea.
Check the application note for a comparison of the 3rd generation Strep-tag® system to His-tag:NiNTA purification under denaturing conditions.
Download experimental data:
Strep-Tactin®XT under denaturing conditions
Download protocol:
Purification with Strep-Tactin®XT under denaturing conditions
Recommended products for high-throughput applications:
The novel high affinity resin Strep-Tactin®XT was sucessfully tested for purification of integral membrane proteins:
Application of Strep-Tactin®XT for affinity purification of Twin-Strep-tagged CB2, a G protein-coupled cannabinoid receptor (Yeliseev et al., 2016)
