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Protein Purification - Application Examples for Strep-Tactin®XT

New applications of the 3rd generation Strep-tag® system

Strep-Tactin®XT:Twin-Strep-tag® is the most versatile affinity purification system and can be used for all kind of applications:
Batch purification

For batch purification IBA provides Strep-Tactin®XT coated magnetic beads: MagStrep "type3" XT beads. MagStrep "type3" XT beads allow the batch purification of highly pure proteins in Strep-tag® quality and the possibility of different elution procedures:

  • Native conditions using biotin
  • Denaturing conditions using sample buffer and boiling for elution
For more information about our MagStrep "type 3" beads click here.
 
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For quick and dirty test purification in batch format you can also use Strep-Tactin®XT Superflow® resin.

Example purification

 

Purification of GFP-Strep-tag fusion protein from crude bact. extract using MagStrep “type3” XT beads

Due to specific binding properties of the beads even the elution by boiling leads to highly pure proteins (GFP peak at ~28kDa ignoring Strep-Tactin XT peak).
(Protein purification analysis was performed with an Agilent Bioanalyzer 2100 system instead of SDS-PAGE)
Purification under denaturing conditions

The 3rd generation Strep-tag® system can now be used for purification under denaturing conditions using up to 6 M urea.

Check the application note for a comparison of the 3rd generation Strep-tag® system to His-tag:NiNTA purification under denaturing conditions.

Download experimental data:

Strep-Tactin®XT under denaturing conditions

Download protocol:

Purification with Strep-Tactin®XT under denaturing conditions

High-throughput screening

Recommended products for high-throughput applications:

Purification of integral membrane proteins with Strep-Tactin®XT

The novel high affinity resin Strep-Tactin®XT was sucessfully tested for purification of integral membrane proteins:

Application of Strep-Tactin®XT for affinity purification of Twin-Strep-tagged CB2, a G protein-coupled cannabinoid receptor (Yeliseev et al., 2016)