6-5000-001
Strep-Tactin® PE
Fluorophore-conjugated Strep-Tactin® for cell staining
Quantity | Unit price |
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1-2 |
€307.50*
|
3-9 |
€292.00*
|
From 10 |
€261.50*
|
Content
Strep-Tactin® conjugated to R-Phycoerythrin (PE) is suitable for staining or isolation of antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®) based on our Strep-tag® technology. To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin®. Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting fluorescent complexes can be used to stain cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents preserves the cells’ full biological activity.
Form: | Suspension in DPBS |
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Possible Application: | Fluorescent cell staining & sorting |
Recommended Usage: | 1 µl per 2 x 10^6 (MHC I-Streps) or 5 x 10^6 (Fab-Streps) total cells |
Storage: | 2-8 °C; Do not freeze! |
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Stability: | 12 months after shipping |
Shipping: | Blue ice |