6-5000-001
Strep-Tactin® PE
Fluorophore-conjugated Strep-Tactin® for cell staining
| Quantity | Unit price |
|---|---|
| 1-2 |
€307.50*
|
| 3-9 |
€292.00*
|
| From 10 |
€261.50*
|
Content
Strep-Tactin® conjugated to R-Phycoerythrin (PE) is suitable for staining or isolation of antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®) based on our Strep-tag® technology. To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin®. Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting fluorescent complexes can be used to stain cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents preserves the cells’ full biological activity.
| Form: | Suspension in DPBS |
|---|---|
| Possible Application: | Fluorescent cell staining & sorting |
| Recommended Usage: | 1 µl per 2 x 10^6 (MHC I-Streps) or 5 x 10^6 (Fab-Streps) total cells |
| Storage: | 2-8 °C; Do not freeze! |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Blue ice |
Global koff-rates of polyclonal T-cell populations merge subclonal avidities and predict functionality (Lückemeier et al., 2022, European Journal of Immunology)
SARS-CoV-2 reactive and neutralizing antibodies discovered by single-cell sequencing of plasma cells and mammalian display (Ehling et al., 2022, Cell Reports)
Protective neutralizing antibodies from human survivors of Crimean-Congo hemorrhagic fever (Fels, J.M. et al. , 2021, Cell)
Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecoviruses (Feldman et al., 2021, Science Immunology)
SLAMF7 and IL-6R define distinct cytotoxic versus helper memory CD8(+) T cells (Loyal et al., 2020, Nature Communications)
Accessories
MHC I-Strep HLA-A*0201; CMV pp65 (NLVPMVATV)
MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide
MHC I-Strep HLA-A*0201; SARS-CoV-2 (YLQPRTFLL)
MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide
CD3 Fab-Strep, human
Low affinity Fab fragment fused to a Twin-Strep-tag® and specific for human CD3
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Gravity flow column suitable for traceless affinity cell and exosome isolation
