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6-5010-001

Strep-Tactin® APC

Fluorophore-conjugated Strep-Tactin® for cell staining
Quantity Unit price
1-2
€355.00*
3-9
€338.00*
From 10
€303.00*
Content
Strep-Tactin® conjugated to allophycocyanin (APC) binds to Strep-tag®II- or Twin-Strep-tag®- fusion proteins and is optimized for flow cytometry of cells. Strep-Tactin® is a streptavidin variant and therefore it is a tetrameric molecule that also has an affinity to biotin.
Cells can either be stained directly if they express a strep-tagged surface protein or indirectly via a strep-tagged protein that subsequently binds to the cells (e.g. for sorting antigen-specific B cells).

Independent from the chosen method, biotin addition will cause the release of Strep-Tactin® APC from the cell surface. This enables the use of selected cells for further downstream applications.

Specifications

Concentration: 75 µg/ml
Form: Suspension in DPBS
Possible Application: Fluorescent cell staining & sorting
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin

Shipping information

Storage: 2-8 °C; Do not freeze!
Stability: 12 months after shipping
Shipping: Blue ice

Accessories

Buffer CI (10x)
Cell isolation buffer containing 10x PBS with 10 mM EDTA and 5% BSA
Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells

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50% suspension for traceless affinity cell and exosome isolation