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5-4043-001
pASG-IBA43 vector
The pASG-IBA43 vector allows the expression of 6x[i]Histidine[/i]-/ [i]Strep[/i]-tag®-fusion-proteins in [i]E.coli[/i]. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the 6x[i]Histidine[/i]-tag for N-terminal and the [i]Strep[/i]-tag® for C-terminal fusion to the recombinant protein as well as the Ampicillin Resistance cassette. It can be used with any [i]E. coli[/i] strain because the tet-promoter works independently from the genetic background of [i]E.coli[/i].
The expressed recombinant protein will be localized in the cytoplasm.
The expressed recombinant protein will be localized in the cytoplasm.
| Affinitätstag (C-terminal): | Strep-tag®II |
|---|---|
| Affinitätstag (N-terminal): | 6xHis |
| Expressionswirt: | E. coli |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor for rekombinante Expression in E. coli |
| Plasmidgröße: | 3910 bp |
| Promoter: | tet Promoter |
| Resistenz: | Ampicillin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
Accessories
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