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StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
- Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coli.
- Optimized for MEXi (Mammalian expression system IBA)
The expressed recombinant protein will be secreted in the cell culture medium (BM40 signal sequence).
This vector does not contain an affinity tag.
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
- Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coli.
- Optimized for MEXi (Mammalian expression system IBA)
The expressed recombinant protein will be secreted in the cell culture medium (BM40 signal sequence).
This vector does not contain an affinity tag.
| Expressionswirt: | Mammaliazellen, MEXi-293E Zellen |
|---|---|
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor für rekombinante Expression in Mammaliazellen |
| Plasmidgröße: | 5771 bp |
| Promoter: | CMV Promoter |
| Resistenz: | Ampicillin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
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