Mammalian expression vector for secretion of N-terminal Twin-Strep-tag® fusion proteins
pDSG-IBA104 is a small transient expression vector encoding an N-terminal Twin-Strep-tag® and was especially developed for the use in combination with the MEXi mammalian expression system. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and the ColE1 replication origin (pUC) for a high plasmid copy number. In addition, it contains the human cytomegalovirus (CMV) immediate-early promoter for high-level expression and the origin of replication from Epstein-Barr Virus (oriP) for extrachromosomal replication driven by EBNA-1 expressed by MEXi-293E cells. The expressed protein is transferred into the medium due to the BM40 secretory signal peptide. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. Please note that cloning into pDSG-IBA acceptor vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called entry vector is possible.
|Affinity Tag N'-terminal:||Twin-Strep-tag®|
|Cloning Method:||Direct cloning using restriction enzyme Esp3I|
|Expression Host:||Mammalian cells, MEXi-293E cells|
|Form:||Suspension in TE buffer|
|Possible Application:||Vector for recombinant expression in mammalian cells|
|Stability:||12 months after shipping|