5-4623-001

pYSG-IBA23 vector

Yeast expression vector encoding an N-terminal GST-tag and C-terminal Strep-tag®II

€219.00*

Content
The pYSG-IBA23 vector is designed for high-level expression of target proteins with an N-terminal GST-tag and C-terminal Strep-tag®II in yeast. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, the ColE1 origin for a high plasmid copy number, the copper-inducible promoter (CUP1) for controlled high-level expression, the URA3 auxotrophy marker for selection after transformation (do not use URA3 for selection during expression), the LEU2d auxotrophy marker for selection to increase plasmid copy number for expression (do not use LEU2d for selection after transformation), and the 2 micron ori for episomal replication in yeast. Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

Affinity Tag C'-terminal: Strep-tag®II
Affinity Tag N'-terminal: GST (removal via PreScission® Protease site)
Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: Yeast
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in yeast
Promoter: CUP1 Promoter
Resistance: Ampicillin
Sequence: See Documents
Size: 8540 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Competent E. coli TOP10
Chemically competent cells for plasmid propagation
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

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pYSG-IBA123 vector
Yeast expression vector encoding an N-terminal GST-tag and C-terminal Twin-Strep-tag®