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Strep-Tactin® for reversible binding of biotinylated or Strep-tagged proteins

Originally the Strep-Tactin® was developed for efficient but reversible binding of a peptide that could be used for protein purification – the Strep-tag®. This Strep-tag®/Strep-Tactin® system is now widely used in the field of protein purification.

Nevertheless, the Strep-Tactin® still has the capability to bind biotin, which is used as specific competitor for elution of strep-tagged proteins. The binding affinity of biotin to Strep-Tactin® is not as high as for streptavidin and therefore biotinylated molecules can bind to Strep-Tactin® in a reversible manner and the release from binding is carried out under mild and physiological conditions by adding an excess of free biotin.

This reversible binding of Strep-Tactin® is applicable for purification, detection and immobilization of biotinylated molecules. Therefore, one example is proximity-dependent biotin identification (BioID).

Strep-Tactin® Products

Bulk order on request: strep-tag@iba-lifesciences.com

Product Contents Cat.no Price   Order
Strep-Tactin®, lyophilized 1 mg 2-1204-001 USD 107.00  
2-1204-001

Strep-Tactin® is a streptavidin variant for optimized Strep-tag®II andTwin-Strep-tag® binding. Nevetheless, it can also reversibly bind biotinylated proteins.

Further details, manuals, data sheets, related products and more
Strep-Tactin®, lyophilized 5 mg 2-1204-005 USD 322.00  
2-1204-005

Strep-Tactin® is a streptavidin variant for optimized Strep-tag®II andTwin-Strep-tag® binding. Nevetheless, it can also reversibly bind biotinylated proteins.

Further details, manuals, data sheets, related products and more

Strep-Tactin® – Product specifications

Form lyophilized from a 25 mg/ml solution in 10 mM potassium phosphate buffer pH 6.5
MW per subunit 13342 Da 
Biotin binding activity > 50 nmol/mg
Purity > 85% as estimated by SDS-PAGE
Reconstitution Dissolve with water 
Stability (lyophilized) 6 months after shipping
Storage (lyophilized) recommended: -25°C to -15°C