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2-5012-001

Strep-Tactin®XT 4Flow® column

Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Quantity Unit price
1-4
€85.00*
From 5
€73.00*
Content

The Strep-Tactin®XT 4Flow gravity flow columns can be used for the purification of proteins that have been tagged with Strep-tag®II or Twin-Strep-tag®. These fusion proteins bind very specifically to the high affinity Strep-Tactin®XT ligand resulting in highly pure target proteins (>95%). It is recommended for challenging and low abundant proteins because of its stability and low agarose concentration. For proteins with increased affinity, like multimeric proteins, Strep-Tactin® resins for easier elution are recommended.
When working with large sample volumes it is recommended to use the application aid WET FRED to reduce the waiting periods during the sample and buffer application.

See our resins specifications for more information and recommendations.

Specifications

Bead Size: 50 - 150 µm spherical
Dynamic Protein Binding Capacity: 5 mg/ml resin
Form: Pre-packed gravity flow column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag® and Strep-tag®II
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

WET FRED Set
Application aid for large volumes of lysates or culture supernatants
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set

Related Products

MagStrep® Strep-Tactin®XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity Spin Column Kit
For rapid purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Empty Spin Columns
For rapid protein purification with Strep-Tactin®XT 4Flow® high capacity
Strep-Tactin®XT 4Flow® FPLC column
FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins