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E. coli expression vectors

E. coli expression vectors are available with a multitude of different features:

Host Promoter Tags
E. coli
Tet
T7
Strep-tag®II,
Twin-Strep-tag®,
6xHis-tag,
FLAG-tag,
GST-tag with PreScission™ (PSC) site

Further special features are available for classic cloning and the respective expression vectors. Read more below.

Detailed information about E. coli expression vectors

StarGate Acceptor Vectors for E. coli

pASG-IBA vectors

  • High-level expression in E. coli, also for toxic proteins
  • Tightly regulated expression due to anhydrotetracycline (AHT) inducible tetA promoter/operator
  • Option for periplasmic expression due to ompA signal sequence
  • No catabolite repression - no influence of medium components
  • Not influenced by the genetic background – wide choice of E. coli expression strains
  • Inexpensive induction with AHT.

pPSG-IBA vectors

  • High-level expression in E. coli by bacteriophage T7 promoter
  • High-level transcription by T7 RNA polymerase in BL21 strains
  • High-level expression of non-toxic proteins
  • Induction by IPTG
  • Suitable for in vitro transcription/translation 
Classic cloning vectors for E. coli

For recombinant protein expression in E. coli, vectors with the Tet promoter (pASK-IBA) are available. The choice of the best expression vector depends on the characteristics of the protein. Due to the identical multiple cloning sites different vector backbones can be easily tested. pASK-IBA vectors are available with different protease cleavage sites (Tev, Factor Xa, Thrombin and Enterokinase) or with chloramphenicol resistance gene.

E. coli expression vector overview

List of E. coli expression vectors and their features
Click to enlarge the figure

 

Expression vector product list for E. coli

Please use filters "Category" and "Subcategory" to quickly select your product from the list below:


Product Contents Cat.no Price   Order
Anhydrotetracycline 50 mg 2-0401-001 USD 159.00  
2-0401-00x

Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ...

Further details, manuals, data sheets, related products and more
Anhydrotetracycline 25 mg 2-0401-002 USD 87.00  
2-0401-00x

Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ...

Further details, manuals, data sheets, related products and more
pASK-IBA6 5 µg 2-1305-000 USD 218.00  
2-1305-000

The pASK-IBA6 vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

Further details, manuals, data sheets, related products and more
pASK-IBA12 5 µg 2-1311-000 USD 218.00  
2-1311-000

The pASK-IBA12 vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

Further details, manuals, data sheets, related products and more
pASK-IBA14 5 µg 2-1313-000 USD 218.00  
2-1313-000

The pASK-IBA14 vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

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pASK-IBA16 5 µg 2-1315-000 USD 218.00  
2-1315-000

The pASK-IBA16 vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

Further details, manuals, data sheets, related products and more
pASK-IBA2C 5 µg 2-1321-000 USD 218.00  
2-1321-000

The pASK-IBA2C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

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pASK-IBA3C 5 µg 2-1322-000 USD 218.00  
2-1322-000

The pASK-IBA3C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

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pASK-IBA4C 5 µg 2-1323-000 USD 218.00  
2-1323-000

The pASK-IBA4C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

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pASK-IBA5C 5 µg 2-1324-000 USD 218.00  
2-1324-000

The pASK-IBA5C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

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pASK-IBA6C 5 µg 2-1325-000 USD 218.00  
2-1325-000

The pASK-IBA6C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

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pASK-IBA7C 5 µg 2-1326-000 USD 218.00  
2-1326-000

The pASK-IBA7C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

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