E. coli expression vectors
E. coli expression vectors are available with a multitude of different features:
| Host | Promoter | Tags |
|---|---|---|
| E. coli |
Tet T7 |
Strep-tag®II, Twin-Strep-tag®, 6xHis-tag, FLAG-tag, GST-tag with PreScission™ (PSC) site |
Further special features are available for classic cloning and the respective expression vectors. Read more below.
Detailed information about E. coli expression vectors
pASG-IBA vectors
- High-level expression in E. coli, also for toxic proteins
- Tightly regulated expression due to anhydrotetracycline (AHT) inducible tetA promoter/operator
- Option for periplasmic expression due to ompA signal sequence
- No catabolite repression - no influence of medium components
- Not influenced by the genetic background – wide choice of E. coli expression strains
- Inexpensive induction with AHT.
pPSG-IBA vectors
- High-level expression in E. coli by bacteriophage T7 promoter
- High-level transcription by T7 RNA polymerase in BL21 strains
- High-level expression of non-toxic proteins
- Induction by IPTG
- Suitable for in vitro transcription/translation
For recombinant protein expression in E. coli, vectors with the Tet promoter (pASK-IBA), protease cleavage site (Factor Xa) and with chloramphenicol resistance gene are available.
E. coli expression vector overview
Expression vector product list for E. coli
Please use filters "Category" and "Subcategory" to quickly select your product from the list below:
| Product | Contents | Cat.no | Price | Order | |||
|---|---|---|---|---|---|---|---|
| Anhydrotetracycline | 50 mg | 2-0401-001 | USD 169.00 | ||||
 Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ... |
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| Anhydrotetracycline | 25 mg | 2-0401-002 | USD 93.00 | ||||
 Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ... |
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| pASK-IBA2C vector | 5 µg | 2-1321-000 | USD 232.00 | ||||
 The pASK-IBA2C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA3C vector | 5 µg | 2-1322-000 | USD 232.00 | ||||
 The pASK-IBA3C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA4C vector | 5 µg | 2-1323-000 | USD 232.00 | ||||
 The pASK-IBA4C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA5C | 5 µg | 2-1324-000 | USD 232.00 | ||||
 The pASK-IBA5C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA6C vector | 5 µg | 2-1325-000 | USD 232.00 | ||||
 The pASK-IBA6C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA7C vector | 5 µg | 2-1326-000 | USD 232.00 | ||||
 The pASK-IBA7C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| Competent cells E. coli TOP10 | 20 rxns | 5-1600-020 | USD 111.00 | ||||
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One-shot chemically competent E.coli TOP10 cells, 20reactions. ... |
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| pASG-IBAwt1 vector | 5 µg | 5-4000-001 | USD 268.00 | ||||
 The pASG-IBAwt1 vector allows the expression of fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the Ampicillin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli. ... |
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| pASG-IBAwt2 vector | 5 µg | 5-4001-001 | USD 268.00 | ||||
 The pASG-IBAwt2 vector allows the expression of fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the Ampicillin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli. ... |
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| pASG-IBA2 vector | 5 µg | 5-4002-001 | USD 268.00 | ||||
 The pASG-IBA2 vector allows the expression of Strep-tag®-fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the Strep-tag® for C-terminal fusion to the recombinant protein and the Ampicillin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli. |
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