E. coli expression vectors
E. coli expression vectors are available with a multitude of different features:
| Host | Promoter | Tags |
|---|---|---|
| E. coli |
Tet T7 |
Strep-tag®II, Twin-Strep-tag®, 6xHis-tag, FLAG-tag, GST-tag with PreScission™ (PSC) site |
Further special features are available for classic cloning and the respective expression vectors. Read more below.
Detailed information about E. coli expression vectors
pASG-IBA vectors
- High-level expression in E. coli, also for toxic proteins
- Tightly regulated expression due to anhydrotetracycline (AHT) inducible tetA promoter/operator
- Option for periplasmic expression due to ompA signal sequence
- No catabolite repression - no influence of medium components
- Not influenced by the genetic background – wide choice of E. coli expression strains
- Inexpensive induction with AHT.
pPSG-IBA vectors
- High-level expression in E. coli by bacteriophage T7 promoter
- High-level transcription by T7 RNA polymerase in BL21 strains
- High-level expression of non-toxic proteins
- Induction by IPTG
- Suitable for in vitro transcription/translation
For recombinant protein expression in E. coli, vectors with the Tet promoter (pASK-IBA) are available. The choice of the best expression vector depends on the characteristics of the protein. Due to the identical multiple cloning sites different vector backbones can be easily tested. pASK-IBA vectors are available with different protease cleavage sites (Tev, Factor Xa, Thrombin and Enterokinase) or with chloramphenicol resistance gene.
E. coli expression vector overview
Expression vector product list for E. coli
Please use filters "Category" and "Subcategory" to quickly select your product from the list below:
| Product | Contents | Cat.no | Price | Order | |||
|---|---|---|---|---|---|---|---|
| Anhydrotetracycline | 50 mg | 2-0401-001 | USD 159.00 | ||||
 Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ... |
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| Anhydrotetracycline | 25 mg | 2-0401-002 | USD 87.00 | ||||
 Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ... |
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| pASK-IBA6 | 5 µg | 2-1305-000 | USD 218.00 | ||||
 The pASK-IBA6 vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA12 | 5 µg | 2-1311-000 | USD 218.00 | ||||
 The pASK-IBA12 vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA14 | 5 µg | 2-1313-000 | USD 218.00 | ||||
 The pASK-IBA14 vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA16 | 5 µg | 2-1315-000 | USD 218.00 | ||||
 The pASK-IBA16 vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA2C | 5 µg | 2-1321-000 | USD 218.00 | ||||
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The pASK-IBA2C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA3C | 5 µg | 2-1322-000 | USD 218.00 | ||||
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The pASK-IBA3C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA4C | 5 µg | 2-1323-000 | USD 218.00 | ||||
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The pASK-IBA4C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA5C | 5 µg | 2-1324-000 | USD 218.00 | ||||
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The pASK-IBA5C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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