Home > Protein Production & Assays > Cloning & Transfection > Cloning > E. coli Expression Vectors - Cloning

E. coli expression vectors

E. coli expression vectors are available with a multitude of different features:

Host Promoter Tags
E. coli
Tet
T7
Strep-tag®II,
Twin-Strep-tag®,
6xHis-tag,
FLAG-tag,
GST-tag with PreScission™ (PSC) site

Further special features are available for classic cloning and the respective expression vectors. Read more below.

Detailed information about E. coli expression vectors

StarGate Acceptor Vectors for E. coli

pASG-IBA vectors

pASG-IBA vectors - E. coli expression vector
  • High-level expression in E. coli, also for toxic proteins
  • Tightly regulated expression due to anhydrotetracycline (AHT) inducible tetA promoter/operator
  • Option for periplasmic expression due to ompA signal sequence
  • No catabolite repression - no influence of medium components
  • Not influenced by the genetic background – wide choice of E. coli expression strains
  • Inexpensive induction with AHT.

pPSG-IBA vectors

pPSG-IBA vectors - E. coli expression vectors
  • High-level expression in E. coli by bacteriophage T7 promoter
  • High-level transcription by T7 RNA polymerase in BL21 strains
  • High-level expression of non-toxic proteins
  • Induction by IPTG
  • Suitable for in vitro transcription/translation 
Classic cloning vectors for E. coli

For recombinant protein expression in E. coli, vectors with the Tet promoter (pASK-IBA), protease cleavage site (Factor Xa) and with chloramphenicol resistance gene are available.

E. coli expression vector overview

List of E. coli expression vectors and their features

 

Expression vector product list for E. coli

Please use filters "Category" and "Subcategory" to quickly select your product from the list below:


Product Contents Cat.no Price   Order
Anhydrotetracycline 50 mg 2-0401-001 USD 164.00  
2-0401-001

Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ...

Further details, manuals, data sheets, related products and more
Anhydrotetracycline 25 mg 2-0401-002 USD 90.00  
2-0401-002

Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ...

Further details, manuals, data sheets, related products and more
pASK-IBA2C vector 5 µg 2-1321-000 USD 225.00  
2-1321-000

The pASK-IBA2C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

Further details, manuals, data sheets, related products and more
pASK-IBA3C vector 5 µg 2-1322-000 USD 225.00  
2-1322-000

The pASK-IBA3C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

Further details, manuals, data sheets, related products and more
pASK-IBA4C vector 5 µg 2-1323-000 USD 225.00  
2-1323-000

The pASK-IBA4C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

Further details, manuals, data sheets, related products and more
pASK-IBA5C 5 µg 2-1324-000 USD 225.00  
2-1324-000

The pASK-IBA5C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

Further details, manuals, data sheets, related products and more
pASK-IBA6C vector 5 µg 2-1325-000 USD 225.00  
2-1325-000

The pASK-IBA6C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

Further details, manuals, data sheets, related products and more
pASK-IBA7C vector 5 µg 2-1326-000 USD 225.00  
2-1326-000

The pASK-IBA7C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ...

Further details, manuals, data sheets, related products and more
Competent cells E. coli TOP10 20 rxns 5-1600-020 USD 108.00  

One-shot chemically competent E.coli TOP10 cells, 20reactions. ...

Further details, manuals, data sheets, related products and more
pASG-IBAwt1 vector 5 µg 5-4000-001 USD 260.00  
5-4000-001

The pASG-IBAwt1 vector allows the expression of fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the Ampicillin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli. ...

Further details, manuals, data sheets, related products and more
pASG-IBAwt2 vector 5 µg 5-4001-001 USD 260.00  
5-4001-001

The pASG-IBAwt2 vector allows the expression of fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the Ampicillin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli. ...

Further details, manuals, data sheets, related products and more
pASG-IBA2 vector 5 µg 5-4002-001 USD 260.00  
5-4002-001

The pASG-IBA2 vector allows the expression of Strep-tag®-fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the Strep-tag® for C-terminal fusion to the recombinant protein and the Ampicillin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli.

Further details, manuals, data sheets, related products and more