E. coli expression vectors
E. coli expression vectors are available with a multitude of different features:
| Host | Promoter | Tags |
|---|---|---|
| E. coli |
Tet T7 |
Strep-tag®II, Twin-Strep-tag®, 6xHis-tag, FLAG-tag, GST-tag with PreScission™ (PSC) site |
Further special features are available for classic cloning and the respective expression vectors. Read more below.
Detailed information about E. coli expression vectors
pASG-IBA vectors
- High-level expression in E. coli, also for toxic proteins
- Tightly regulated expression due to anhydrotetracycline (AHT) inducible tetA promoter/operator
- Option for periplasmic expression due to ompA signal sequence
- No catabolite repression - no influence of medium components
- Not influenced by the genetic background – wide choice of E. coli expression strains
- Inexpensive induction with AHT.
pPSG-IBA vectors
- High-level expression in E. coli by bacteriophage T7 promoter
- High-level transcription by T7 RNA polymerase in BL21 strains
- High-level expression of non-toxic proteins
- Induction by IPTG
- Suitable for in vitro transcription/translation
For recombinant protein expression in E. coli, vectors with the Tet promoter (pASK-IBA), protease cleavage site (Factor Xa) and with chloramphenicol resistance gene are available.
E. coli expression vector overview
Expression vector product list for E. coli
Please use filters "Category" and "Subcategory" to quickly select your product from the list below:
| Product | Contents | Cat.no | Price | Order | |||
|---|---|---|---|---|---|---|---|
| Anhydrotetracycline | 50 mg | 2-0401-001 | USD 164.00 | ||||
 Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ... |
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| Anhydrotetracycline | 25 mg | 2-0401-002 | USD 90.00 | ||||
 Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ... |
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| pASK-IBA2C | 5 µg | 2-1321-000 | USD 225.00 | ||||
 The pASK-IBA2C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA3C | 5 µg | 2-1322-000 | USD 225.00 | ||||
 The pASK-IBA3C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA4C | 5 µg | 2-1323-000 | USD 225.00 | ||||
 The pASK-IBA4C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA5C | 5 µg | 2-1324-000 | USD 225.00 | ||||
 The pASK-IBA5C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA6C | 5 µg | 2-1325-000 | USD 225.00 | ||||
 The pASK-IBA6C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| pASK-IBA7C | 5 µg | 2-1326-000 | USD 225.00 | ||||
 The pASK-IBA7C vector allows the expression of Strep-tag®-fusion-proteins in E.coli. ... |
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| Forward sequencing primer for pASG-IBA and pASK-IBA vectors (HPLC purified) | 1 nmol | 5-0000-101 | USD 26.00 | ||||
| Reverse sequencing primer for pASG-IBA and pASK-IBA vectors (HPLC-purified) | 1 nmol | 5-0000-102 | USD 26.00 | ||||
| Forward and Reverse sequencing primers for pASG-IBA and pASK-IBA vectors (HPLC-purified) | 1 nmol each | 5-0000-104 | USD 34.00 | ||||
| Forward sequencing primer for pPSG-IBA vectors (HPLC-purified) | 1 nmol | 5-0000-111 | USD 26.00 | ||||
