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2-1613-002

MagStrep® Strep-Tactin® beads

Magnetic beads (5% suspension) to purify biotinylated proteins

€230.00*

Content: 1 Stk
Content
The MagStrep® Strep-Tactin® beads are an ideal tool for the isolation of biotinylated and strep-tagged proteins or identification of complexes via pull-downs, immunoprecipitation (IP), or co-immunoprecipitation (Co-IP) in batch format and high-throughput screenings.
For cell isolation we offer magnetic microbeads with a smaller diameter. We also recommend our MagStrep® Strep-Tactin®XT beads if you need increased binding affinity in the pM range for protein purification.
The advantage of Strep-Tactin® over native streptavidin is that it binds to biotinylated proteins in a reversible manner. The binding can be released by adding biotin in excess. As streptavidin binds irreversibly to biotinylated proteins, they cannot be eluted. Strep-Tactin® however still binds biotin and therefore also biotinylated proteins, but with a lower binding affinity compared to streptavidin, making the binding reversible.
Separation of beads is easy with a magnetic separator. Elution of strep-tagged or biotinylated proteins can be done by using buffer BXT containing biotin for purification under physiological conditions or under denaturing conditions by boiling in SDS gel loading buffer for elution.

Specifications

Bead Size: 30 µm (average)
Form: 5% suspension
Possible Application: Purification of biotinylated proteins
Specificity: Biotinylated proteins, Twin-Strep-tag® and Strep-tag®II
Strep-tag®II Binding Capacity: 7.5 mg/ml
Support: 6% agarose, crosslinked, spherical magnetic beads
Theoretical biotin binding capacity: 3 nmol/µl beads

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Magnetic Separator
Device for separation of magnetic beads from residual solution
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer

Related Products

Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins