MagStrep “type3” XT beads consist of a magnetic core coated with a 6% cross-linked agarose that is conjugated with the streptavidin variant Strep-Tactin®XT
. They can be used for the purification of Strep-tag®II and Twin-Strep-tag®
fusion proteins. The beads can be easily and swiftly separated with a magnetic separator
, eliminating the need for centrifugation and reducing the risk of disturbing the pellet or loss of beads within the wash step to zero.
As with any other Strep-Tactin®XT resin, extremely low unspecific protein binding results in an outstanding purity of the target protein. Combined with the highest binding capacity among all our Strep-Tactin®XT resins, this makes MagStrep “type3” XT beads a perfect tool for isolation of proteins or complexes via pull-downs, immunoprecipitation (IP), or co-immunoprecipitation (Co-IP) in batch format and/or high-throughput screenings.
The elution of target proteins or complexes can be triggered under mild conditions with the biotin-containing Buffer BXT or under denaturing conditions by boiling in SDS gel loading buffer. 20 μl suspension correspond to 1 μl magnetic beads.