Specifications for Strep-Tactin® Purification Resins
There are several Strep-Tactin® resin versions available. All resins differ in their properties and suitability for applications. Learn more about the different resin matrices in order to know when to use which resin.
|(only available as 20 ml slurry)|
|High capacity available||yes||no||no||yes||yes|
|Gravity flow column||yes||yes||yes||yes||yes|
|Binding capacity*||classic:3 mg/ml resin||3 mg/ml resin||3 mg/ml resin||classic: n.d.||classic: 6.5 mg/ml resin|
|high capacity: n.d.||high capacity: n.d.||high capacity: 11.8 mg/ml resin|
|Dynamic binding capacity**||classic: n.d.||n.d.||n.d.||classic: 4.1 mg/ml resin||classic: 7 mg/ml resin|
|high capacity: 7.0 mg/ml resin||high capacity: 15 mg/ml resin||high capacity: 16 mg/ml resin|
|Bead structure||6% agarose, crosslinked||4% agarose, crosslinked||polymethacrylate||6% agarose, crosslinked||4% agarose, highly crosslinked|
|Bead size||60-160 µm, spherical||45-165 µm||50 µm||60-160 µm, spherical||50-150 µm, spherical|
|Exclusion limit||6 x 106||~3 x 107||1 x 106||6 x 106||3 x 107|
|Recommended flow rate||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min|
|pH range for protein binding||7-8||7-8||7-8||6-10||4-10|
|Max pressure||9.6 bar||gravity flow||70 bar||9.6 bar||9.6 bar|
|Storage||4 °C, do not freeze||4 °C, do not freeze||4 °C, do not freeze||4 °C, do not freeze||4 °C, do not freeze|
|Regeneration buffer||Buffer R||Buffer R||Buffer R||Buffer XT-R||Buffer XT-R|
|Features||The Strep-Tactin® Superflow high capacity binds in comparison to Strep-Tactin® Superflow a three to five fold higher amount of target protein and thereby achieves a higher yield.||Strep-Tactin® Sepharose is a good alternative for large proteins, if the affinity of Strep-Tactin®XT 4Flow® is too high.||The alternative when cell components (e.g. chlorophyll from plant extracts) bind non-specifically to Sepharose, Superflow or 4Flow®.||Strep-Tactin®XT Superflow high capacity achieves higher yields than Strep-Tactin® Superflow high capacity and thereby is more cost efficient.||Strep-Tactin®XT 4Flow® is the resin to start with. Due to the higher affinity compared to Strep-Tactin® resins it is particularly applicable for low abundant or challenging proteins.|
|If the affinity of Strep-Tactin®XT resins is too high and the target protein is not fully eluted, then Strep-Tactin® Superflow is a good alternative.||Only suitable for gravity flow chromatography due to the low pressure stability.||Synthetic support in comparison to all other resins.||Can be used for protein purification under denaturing conditions (6 M urea).||Suitable for all types of proteins and especially for large proteins, because it has the high affinity of Strep-Tactin®XT, the broadest pH stability and consists of a low-concentrated agarose.|
* Max binding capacity for a Strep-tag® protein (30kDa).
** Dynamic binding capacity (DBC) was determined with mCherry-Twin-Strep-tag® (30 kDa). The DBC is determined under realistic operation conditions and shows the amount of protein which is bound until 10% of the protein is found in the flow through.
For purification of Strep-tag®II proteins by gravity flow column chromatography
For packing individual columns bulk material is available.
For batch purification we recommend to use magnetic beads: MagStrep "type3" XT beads.
Strep-Tactin® Superflow® resin:
- combines outstanding flow characteristics with superior mechanical stability
- is suitable for increased flow rates and useful for purification of large protein complexes
- yields much higher protein purity (> 95%) than 6xHistidine-tag purification on Ni-NTA Superflow®
Strep-Tactin® Superflow® resin is available as:
- 50% suspension (slurry)
Strep-Tactin® Superflow® high capacity resin offers a three times higher binding capacity at identical high protein purity. It is the solution for Strep-tag® fusion proteins at high expression levels.
Strep-Tactin® Superflow® high capacity resin is available as:
50% suspension (slurry)
pre-packed Strep-Tactin® gravity flow columns
pre-packed cartridges for work with automated workstations like Äkta
So far, Strep-Tactin® Superflow® high capacity is one of the most favored resins allowing the purification of recombinant proteins in high yields. However, the new Strep-Tactin®XT Superflow® high capacity provides significant more purified protein because it combines an increased capacity of the resin with a very efficient binding due to the high affinity for Strep-tag®II and Twin-Strep-tag®.
The MacroPrep® resin exhibits non-specific binding properties differing from those of Sepharose® or Superflow® and can, thus, be recommended in cases where suboptimal results are obtained with Sepharose® or Superflow®, and vice versa.
MacroPrep® is available in one format:
- 50% suspension (slurry)