Strep-Tactin® vs Strep-Tactin®XT
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What are the differences?
The Streptavidin:Biotin binding is the strongest non-covalent biological interaction known. With IBA's newly developed Strep-Tactin®XT in combination with Twin-Strep-tag®, this near covalent binding affinity can be used for e.g. protein purification, protein interaction analysis or assays (e.g. BIAcore). Since Biotin is still binding strongly to Strep-Tactin®XT it can be used for elution of Twin-Strep-tag® fusion proteins.
µM for Strep-tag®
nM for Twin-Strep-tag®
nM for Strep-tag®
pM for Twin-Strep-tag®
|pH range||7.5 - 9||5 - 10|
|Denaturing conditions||no||yes (up to 6 M urea)|
|Ease of use||yes||yes|
|Regeneration condition||HABA (Buffer R)||NaOH|
Benefits of Strep-Tactin®XT
Strep-Tactin®XT allows the purification of higher protein yields compared to Strep-Tactin®. This was determined by purification of different target protein on either Strep-Tactin® or Strep-Tactin®XT. The obtained protein yields were determined and compared in the graph below. Here, we found that Strep-Tactin®XT allows in average the purification of a 2-fold higher protein yield compared to Strep-Tactin®.
An advantage of the new Strep-tag® system III is that it allows intensive washing without the loss in protein yield. This figure shows the purification of mCherry-Twin-Strep-tag® fusion protein via 1 ml Strep-Tactin® Superflow® resin and 1 ml Strep-Tactin®XT Superflow® resin. E. coli supernatants were prepared and applied onto the column. Then the columns were washed with 8 CV Buffer W in order to reach a constant absorption at A260/A280 nm. Then the target protein was eluted with 50 mM biotin. Samples of the last wash fraction and the elution fraction were analysed on SDS-PAGE showing that some of the target protein was washed down from Strep-Tactin® Superflow® resin, whereas no protein was eluted from Strep-Tactin®XT Superflow® resin.
The elution fractions were further analysed in Western Blot, showing that the remaining bands are degradation products of mCherry.
The following assay shows the comparison of the immobilization efficiency of bacterial alkaline phosphatase (BAP-Strep-tag®II) on Strep-Tactin®XT and Strep-Tactin®. Here, different amounts of BAP were subjected to the coated microplates. After washing, the relative maximum amount of immobilized BAP was determined. The relative signal intensity reflects the amount of bound BAP.
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Get an overview on the system components
The Strep-tag® system is based on one of the strongest non-covalent interactions in nature, which is the interaction of biotin to streptavidin. It allows the purification, detection and immobilization of highly pure and bioactive recombinant proteins. The system includes two affinity tags: Strep-tag®II is a synthetic peptide consisting of eight amino acids and Twin-Strep-tag® consists of two Strep-tag®II sequences in series combined by a GS-linker. This peptide sequences exhibit intrinsic affinity towards Strep-Tactin® and Strep-Tactin®XT, two specifically engineered streptavidin variants.