Immobilization with Strep-Tactin®XT:Twin-Strep-tag®
Due to the extra tight binding affinity of Strep-Tactin®XT to Twin-Strep-tag®, the system is highly suitable for efficient immobilization of Twin-Strep-tag® (and Strep-tag®II) fusion proteins.
The binding affinity is in the range of antibody based immobilization systems, but more economic and straight-forward to use.
Key benefits of Strep-Tactin®XT and Twin-Strep-tag® for immobilization experiments
- High affinity in pM range (T1/2 = 13 h)
- Reversibility due to addition of Biotin
- Minimal non-specific binding