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Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set
This buffer set contains all buffers necessary for purifying Strep-tag®II or Twin-Strep-tag® fusion proteins with Strep-Tactin®XT and for subsequent resin regeneration.
10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin® and Strep-Tactin®XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag®II and Twin-Strep-tag® fusion protein purification. 10x Buffer BXT is a 10x concentrated Strep-Tactin®XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin®XT with a very high affinity, but reversibly, so Strep-tag®II or Twin-Strep-tag® proteins can be eluted efficiently from resins. Please note that 10x Buffer BXT is not applicable for elution from Strep-Tactin® resins, since biotin irreversibly binds to these resins and Strep-Tactin® cannot be regenerated. Buffer XT-R is a ready-to-use Strep-Tactin®XT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-Tactin®XT after the elution of Strep-tag®II or Twin-Strep-tag® fusion proteins.
Please note that the Strep-Tactin®XT Buffer Set is not applicable for Strep-Tactin®.
10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin® and Strep-Tactin®XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag®II and Twin-Strep-tag® fusion protein purification. 10x Buffer BXT is a 10x concentrated Strep-Tactin®XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin®XT with a very high affinity, but reversibly, so Strep-tag®II or Twin-Strep-tag® proteins can be eluted efficiently from resins. Please note that 10x Buffer BXT is not applicable for elution from Strep-Tactin® resins, since biotin irreversibly binds to these resins and Strep-Tactin® cannot be regenerated. Buffer XT-R is a ready-to-use Strep-Tactin®XT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-Tactin®XT after the elution of Strep-tag®II or Twin-Strep-tag® fusion proteins.
Please note that the Strep-Tactin®XT Buffer Set is not applicable for Strep-Tactin®.
| Concentration: | 10x concentrated |
|---|---|
| Form: | Solution |
| Kit Content: | 100 ml 10x Buffer W, 25 ml 10x Buffer BXT, 250 ml Buffer XT-R |
| Possible Application: | Protein purification with Strep-Tactin®XT resins |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® FPLC column
FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
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