2-1043-000

Strep-Tactin®XT Buffer Set

Strep-Tactin®XT purification buffer set

€85.00*

Content
Strep-Tactin®XT Buffer Set contains all buffers necessary for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins with Strep-Tactin®XT as well as for subsequent regeneration of the resin. 10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin® and Strep-Tactin®XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag®II and Twin-Strep-tag® fusion protein purification. 10x Buffer BXT is a 10x concentrated Strep-Tactin®XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin®XT with very high affinity but reversibly and, thereby, allows the efficient elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from the resin. Please note that 10x Buffer BXT is not applicable for elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from Strep-Tactin®, since biotin irreversibly binds to these resins, and, thus, Strep-Tactin® cannot be regenerated. Buffer XT-R is a ready-to-use Strep-Tactin®XT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-Tactin®XT after the elution of Strep-tag®II or Twin-Strep-tag® fusion proteins. Please note that the Strep-Tactin®XT Buffer Set is not applicable for Strep-Tactin®.

Specifications

Concentration: 10x concentrated
Form: Solution
Kit Content: 100 ml 10x Buffer W, 25 ml 10x Buffer BXT, 250 ml Buffer XT-R
Possible Application: Protein purification with Strep-Tactin®XT resins

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® cartridge
HPLC/FPLC cartridge to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity cartridge
HPLC/FPLC cartridge to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Related Products

Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
10x Buffer BXT
Strep-Tactin®XT elution buffer
10x Buffer W
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer