Buffers & Reagents for Protein Purification with Strep-Tactin®
Product | Contents | Cat.no | Price | Order | |||
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Avidin | 15 mg | 2-0204-015 | USD 137.00 | ||||
Avidin prevents biotinylated proteins from binding to Strep-Tactin® or Strep-Tactin XT columns during Twin-Strep-tag and Strep-tag® protein purification. ... |
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Avidin | 50 mg | 2-0204-050 | USD 270.00 | ||||
Avidin prevents biotinylated proteins from binding to Strep-Tactin® or Strep-Tactin XT columns during Twin-Strep-tag and Strep-tag® protein purification. ... |
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BioLock | 50 ml | 2-0205-050 | USD 185.00 | ||||
Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media. ... |
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BioLock | 250 ml | 2-0205-250 | USD 747.00 | ||||
Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media. ... |
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Anhydrotetracycline | 50 mg | 2-0401-001 | USD 169.00 | ||||
Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ... |
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Anhydrotetracycline | 25 mg | 2-0401-002 | USD 93.00 | ||||
Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ... |
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Desthiobiotin | 0.107 g | 2-1000-001 | USD 66.00 | ||||
Desthiobiotin for elution of Strep-tag® II proteins from Strep-Tactin®. It displaces Strep-tag® II proteins at the biotin-binding site of Strep-Tactin® in a competitive manner resulting in a mild elution. ... |
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Desthiobiotin | 1 g | 2-1000-002 | USD 205.00 | ||||
Desthiobiotin for elution of Strep-tag® II proteins from Strep-Tactin®. It displaces Strep-tag® II proteins at the biotin-binding site of Strep-Tactin® in a competitive manner resulting in a mild elution. ... |
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Desthiobiotin | 5 g | 2-1000-005 | USD 904.00 | ||||
Desthiobiotin for elution of Strep-tag® II proteins from Strep-Tactin®. It displaces Strep-tag® II proteins at the biotin-binding site of Strep-Tactin® in a competitive manner resulting in a mild elution. ... |
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10x Buffer E | 25 ml | 2-1000-025 | USD 66.00 | ||||
Buffer E is a Strep-Tactin® Elution Buffer with Desthiobiotin (10x concentrated) and used for elution of Strep-tag®II and Twin-Strep-tag® proteins from the biotin-binding site of Strep-Tactin® resins in a competitive manner resulting in a mild elution. ... |
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Strep-Tactin® Buffer Set | 1 set | 2-1002-001 | USD 93.00 | ||||
Strep-Tactin® Purification Buffer Set is a convenient buffer set for the purification of recombinant Strep-tag®II and Twin-Strep-tag®II fusion proteins on immobilized Strep-Tactin® and its regeneration. ... |
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10x Buffer R | 100 ml | 2-1002-100 | USD 54.00 | ||||
Buffer R is a Strep-Tactin® Regeneration Buffer with HABA for the regeneration of immobilized Strep-Tactin®. HABA (2-[4 -hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin (present in Buffer E: Strep-Tactin® Elution Buffer with Desthiobiotin) at the biotin-binding site of Strep-Tactin®. The yellow HABA solution turns red upon binding to Strep-Tactin® clearly indicating the regeneration process and activity status of the column. ... |
Read about the provided Buffers & Reagents:
For removal of unbound proteins and other material during Strep-tag® protein purification via Strep-Tactin® and Strep-Tactin®XT columns.
Desthiobiotin displaces Strep-tag®II proteins at the biotin-binding site of Strep-Tactin® in a competitive manner resulting in a mild elution of the protein. For your convenience, we offer desthiobiotin ready-to-use in solution (Buffer E). If you wish to prepare your own elution buffer, desthiobiotin is also offered as a lyophilized powder. Please note that after elution with Buffer E, the Strep-Tactin® column can be regenerated for atleast 3-5 times with HABA.
Do not use in combination with Strep-Tactin®XT!!!
Contains HABA (2-[4'-hydroxy-benzeneazo]benzoic acid), which displaces desthiobiotin at the biotin-binding site regenerating Strep-Tactin®. The yellow HABA solution turns red when desthiobiotin has been removed from Strep-Tactin®, clearly indicating the regeneration process and activity status of the column.
Do not use in combination with Strep-Tactin®XT!!!
Convenient set for the purification of recombinant Strep-tag® fusion proteins on immobilized Strep-Tactin® and for the regeneration of the columns. Consists of Buffers W / E / R.
Do not use in combination with Strep-Tactin®XT!!!
Biotin is often present in cell culture media. Its nearly irreversible binding to Strep-Tactin® has to be taken into account when recombinant proteins, secreted to the cell culture supernatant, are aimed to be directly purified via Strep-Tactin® affinity chromatography for two reasons:
- It efficiently competes with the (Twin-)Strep-tag® target protein, thereby preventing it from binding to the resin
- it inactivates Strep-Tactin® resins and thus, makes re-use impossible.
BioLock is a ready-to-use and cost effective
solution for the removal of free biotin from cell culture supernatant.
It simply has to be added to the cell culture supernatant prior to
purification.
The required amount of BioLock for effective biotin blocking can
easily be determined since 1ml BioLock blocks 70 μg biotin. In addition
we provide a table with the required BioLock amounts for standard
mammalian and insect cell culture media at
www.iba-lifesciences.com/technical-support.html >> troubleshooting>> Biotin Blocking
Is used for induction of tet promoter controlled pASK-IBA vectors. The E. coli expression cassette of the Strep-tag®/Strep-Tactin® overexpression system is under transcriptional control of the tetA promoter/operator and repressor. The promoter is induced by a low concentration of anhydrotetracycline (AHT) saving costs and minimizing the antibacterial influence of AHT.
Prevents biotin and biotinylated proteins from binding to Strep-Tactin® columns during Strep-tag® protein purification. This is important if growth media containing free Biotin are used (for further information see “Biotin Blocking”).
For blocking biotinylated proteins in Western blots (like BCCP protein from E. coli), the membrane is incubated with Biotin Blocking Buffer. Use a dilution of 1:1000 in standard Western blot blocking reagent prior to detecting Strep-tag® proteins with Strep-Tactin® conjugates.
For specific customer purposes.