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Buffers & Reagents for Protein Purification with Strep-Tactin®

Product Contents Cat.no Price   Order
Avidin 15 mg 2-0204-015 USD 111.00  
2-0204-0xx

Avidin prevents biotinylated proteins from binding to Strep-Tactin® or Strep-Tactin XT columns during Twin-Strep-tag and Strep-tag® protein purification.

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Avidin 50 mg 2-0204-050 USD 218.00  
2-0204-0xx

Avidin prevents biotinylated proteins from binding to Strep-Tactin® or Strep-Tactin XT columns during Twin-Strep-tag and Strep-tag® protein purification.

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Anhydrotetracycline 50 mg 2-0401-001 USD 159.00  
2-0401-00x

Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ...

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Anhydrotetracycline 25 mg 2-0401-002 USD 87.00  
2-0401-00x

Anhydrotetracycline for induction of tet promoter controlled pASK-IBA and pASG-IBA vectors. ...

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Biotin Blocking Buffer 2 ml 2-0501-002 USD 78.00  
2-0501-002

Biotin Blocking Buffer is recommended for blocking biotinylated proteins on Western blots prior to detection of Strep-tag® proteins with the Strep-Tactin® AP conjugate or Strep-Tactin® HRP conjugate.

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Desthiobiotin 0.107 g 2-1000-001 USD 62.00  
2-1000-001

Desthiobiotin for elution of Strep-tag® II proteins from Strep-Tactin®. It displaces Strep-tag® II proteins at the biotin-binding site of Strep-Tactin® in a competitive manner resulting in a mild elution. ...

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Desthiobiotin 1 g 2-1000-002 USD 193.00  
2-1000-002

Desthiobiotin for elution of Strep-tag® II proteins from Strep-Tactin®. It displaces Strep-tag® II proteins at the biotin-binding site of Strep-Tactin® in a competitive manner resulting in a mild elution. ...

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Desthiobiotin 5 g 2-1000-005 USD 852.00  
2-1000-005

Desthiobiotin for elution of Strep-tag® II proteins from Strep-Tactin®. It displaces Strep-tag® II proteins at the biotin-binding site of Strep-Tactin® in a competitive manner resulting in a mild elution. ...

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10x Buffer E; Strep-Tactin® Elution Buffer with Desthiobiotin 25 ml 2-1000-025 USD 62.00  
2-1000-025

Buffer E is a Strep-Tactin® Elution Buffer with Desthiobiotin (10x concentrated) and used for elution of Strep-tag®II and Twin-Strep-tag® proteins from the biotin-binding site of Strep-Tactin® resins in a competitive manner resulting in a mild elution. ...

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Strep-Tactin® Purification Buffer Set 1 Set 2-1002-001 USD 87.00  
2-1002-001

Strep-Tactin® Purification Buffer Set is a convenient buffer set for the purification of recombinant Strep-tag®II and Twin-Strep-tag®II fusion proteins on immobilized Strep-Tactin® and its regeneration. ...

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Buffer R; Strep-Tactin® Regeneration Buffer with HABA; 100 ml 2-1002-100 USD 50.00  
2-1002-100

Buffer R is a Strep-Tactin® Regeneration Buffer with HABA for the regeneration of immobilized Strep-Tactin®. HABA (2-[4 -hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin (present in Buffer E: Strep-Tactin® Elution Buffer with Desthiobiotin) at the biotin-binding site of Strep-Tactin®. The yellow HABA solution turns red upon binding to Strep-Tactin® clearly indicating the regeneration process and activity status of the column. ...

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10x Buffer W; Strep-Tactin®/Strep-Tactin®XT Wash Buffer 100 ml 2-1003-100 USD 43.00  
2-1003-100

Strep-Tactin®/Strep-Tactin®XT Wash Buffer is used for the removal of unbound molecules from immobilized Strep-Tactin® and Strep-Tactin®XT during Strep-tag® protein purification.

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Read about the provided Buffers & Reagents:

Buffer W: Strep-Tactin®/Strep-Tactin®XT Wash Buffer

For removal of unbound proteins and other material during Strep-tag® protein purification via Strep-Tactin® and Strep-Tactin®XT columns.

Buffer E: Strep-Tactin® Elution Buffer with Desthiobiotin

Desthiobiotin displaces Strep-tag®II proteins at the biotin-binding site of Strep-Tactin® in a competitive manner resulting in a mild elution of the protein. For your convenience, we offer desthiobiotin ready-to-use in solution (Buffer E). If you wish to prepare your own elution buffer, desthiobiotin is also offered as a lyophilized powder. Please note that after elution with Buffer E, the Strep-Tactin® column can be regenerated for atleast 3-5 times with HABA.

Do not use in combination with Strep-Tactin®XT!!!

Buffer R: Strep-Tactin® Regeneration Buffer with HABA

Contains HABA (2-[4'-hydroxy-benzeneazo]benzoic acid), which displaces desthiobiotin at the biotin-binding site regenerating Strep-Tactin®. The yellow HABA solution turns red when desthiobiotin has been removed from Strep-Tactin®, clearly indicating the regeneration process and activity status of the column.

Do not use in combination with Strep-Tactin®XT!!!

Strep-Tactin® Purification Buffer Set

Convenient set for the purification of recombinant Strep-tag® fusion proteins on immobilized Strep-Tactin® and for the regeneration of the columns. Consists of Buffers W / E / R.

Do not use in combination with Strep-Tactin®XT!!!

BioLock - Biotin Blocking Solution

Biotin is often present in cell culture media. Its nearly irreversible binding to Strep-Tactin® has to be taken into account when recombinant proteins, secreted to the cell culture supernatant, are aimed to be directly purified via Strep-Tactin® affinity chromatography for two reasons:

  • It efficiently competes with the (Twin-)Strep-tag® target protein, thereby preventing it from binding to the resin
  • it inactivates Strep-Tactin® resins and thus, makes re-use impossible.


BioLock is a ready-to-use and cost effective solution for the removal of free biotin from cell culture supernatant. It simply has to be added to the cell culture supernatant prior to purification.

The required amount of BioLock for effective biotin blocking can easily be determined since 1ml BioLock blocks 70 μg biotin. In addition we provide a table with the required BioLock amounts for standard mammalian and insect cell culture media at
www.iba-lifesciences.com/technical-support.html >> troubleshooting>> Biotin Blocking

Buffer BE: Strep-Tactin® Regeneration Buffer with Biotin

Elution buffer for competitive displacement of purified recombinant Strep-tag® or Twin-Strep-tag® fusion proteins from immobilized Strep-Tactin®. The buffer is recommended for the elution from Strep-Tactin® Spin columns.
This buffer is also recommended for use with Twin-Strep-tag® in protein complex or PPI applications, since it contains Biotin, which has a higher affinity to Strep-Tactin® and therefore, more efficiently competes with the (Twin)Strep-tag® binding. The regeneration of the Strep-Tactin® columns in PPI applications is not recommended in order to avoid cross contaminations.

Please note that after elution with Buffer BE the resin cannot be regenerated.

Do not use in combination with Strep-Tactin®XT!!!

Anhydrotetracycline

Is used for induction of tet promoter controlled pASK-IBA vectors. The E. coli expression cassette of the Strep-tag®/Strep-Tactin® overexpression system is under transcriptional control of the tetA promoter/operator and repressor. The promoter is induced by a low concentration of anhydrotetracycline (AHT) saving costs and minimizing the antibacterial influence of AHT.

Avidin

Prevents biotin and biotinylated proteins from binding to Strep-Tactin® columns during Strep-tag® protein purification. This is important if growth media containing free Biotin are used (for further information see “Biotin Blocking”).

Biotin Blocking Buffer (Western blots)

For blocking biotinylated proteins in Western blots (like BCCP protein from E. coli, see Applications), the membrane is incubated with Biotin Blocking Buffer. Use a dilution of 1:1000 in standard Western blot blocking reagent prior to detecting Strep-tag® proteins with Strep-Tactin® conjugates.

Strep-tag®II Peptide

For specific customer purposes.