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high binding capacity (up to 0.85 nmol/μl beads, corresponding to 30 μg of a 30 kDa protein)
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very low non-specific protein binding due to an improved coating
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flexible elution conditions (under denaturing conditions by boiling in SDS gel loading buffer or under native conditions with biotin)
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high affinity for Twin-Strep-tag® – due to new Strep-Tactin®XT
These characteristics lead to very good purification results and make the MagStrep “type3” XT beads a superior tool for small scale purifications with high demands on protein purity and high-throughput screenings.
| Product | Contents | Cat.no | Price | Order | |||
|---|---|---|---|---|---|---|---|
| 10x Buffer W; Strep-Tactin®/Strep-Tactin®XT Wash Buffer | 100 ml | 2-1003-100 | USD 43.00 | ||||
 Strep-Tactin®/Strep-Tactin®XT Wash Buffer is used for the removal of unbound molecules from immobilized Strep-Tactin® and Strep-Tactin®XT during Strep-tag® protein purification. |
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| Biotin | 2 g | 2-1016-002 | USD 74.00 | ||||
 Biotin is a water-soluble B-complex vitamin (vitamin B7 or vitamin H) ... |
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| Biotin | 5 g | 2-1016-005 | USD 159.00 | ||||
 Biotin is a water-soluble B-complex vitamin (vitamin B7 or vitamin H) ... |
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| 10x Buffer BXT; Strep-Tactin®XT Elution Buffer | 25 ml | 2-1042-025 | USD 62.00 | ||||
 *** This article replaces 2-1041-250, 1x Buffer BXT *** ... |
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| Magnetic Separator | for 24 reaction tubes | 2-1602-000 | USD 225.00 | ||||
 Magnetic separator (for 24 reaction tubes, 12x 1.5 ml and 12x 2 ml) for the fast purification of Strep-tag® proteins with MagStrep "type3" XT beads (Strep-Tactin®XT coated magnetic beads) in batch format. Within minutes, the target molecules are bound specifically by Strep-Tactin®XT. Using the magnetic separator, the magnetic beads are separated within seconds from solution. |
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| MagStrep "type3" XT beads 5% suspension | 2 ml | 2-4090-002 | USD 200.00 | ||||
References
See where to use MagStrep “type 3” XT beads!
- Protein purification
Essuman K, Summers DW, Sasaki Y, Mao X, Yim AKY, DiAntonio A, Milbrandt J. (2018) TIR Domain Proteins Are an Ancient Family of NAD+ -Consuming Enzymes. Curr Biol. 28(3): 421-430 [Link]
Zhang Y, Ptacin JL, Fischer EC, Aerni HR, Caffaro CE, San Jose K, Feldman AW, Turner CR, Romesberg FE. (2017) A semi-synthetic organism that stores and retrieves increased genetic information. Nature. 551(7682): 644-647. [Link]
- Pull-down assay
González M, Brito N, González C. (2017) The Botrytis cinerea elicitor protein BcIEB1 interacts with the tobacco PR5-family protein osmotin and protects the fungus against its antifungal activity. New Phytol. 215(1): 397-410. [Link]
- RNA co-immunoprecipitation
Chen Z, Mashburn-Warren L, Merritt J, Federle MJ, Kreth J. (2017) Interference of a speB 5' untranslated region partial deletion with mRNA degradation in Streptococcus pyogenes. Mol Oral Microbiol. 32(5): 390-403. [Link]
