MagStrep "type3" XT beads

 

 

Technology

Magnetic Strep-tag® purification at its best! - A superior tool for small scale purifications with high demands on protein purity. MagStrep “type3” XT beads are Strep-Tactin®XT coated ferri-magnetic spheres with the following characteristics:
  • high binding capacity (up to 0.85 nmol/μl beads, corresponding to 30 μg of a 30 kDa protein)
  • very low non-specific protein binding due to an improved coating
  • flexible elution conditions (under denaturing conditions by boiling in SDS gel loading buffer or under native conditions with biotin)
  • high affinity for Twin-Strep-tag® – due to new Strep-Tactin®XT

These characteristics lead to very good purification results and make the MagStrep “type3” XT beads a superior tool for small scale purifications with high demands on protein purity and high-throughput screenings.

Products

Product Contents Cat.no Price   Order
10x Buffer W; Strep-Tactin®/Strep-Tactin®XT Wash Buffer 100 ml 2-1003-100 USD 43.00  
2-1003-100

Strep-Tactin®/Strep-Tactin®XT Wash Buffer is used for the removal of unbound molecules from immobilized Strep-Tactin® and Strep-Tactin®XT during Strep-tag® protein purification.

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Biotin 2 g 2-1016-002 USD 74.00  
2-1016-002

Biotin is a water-soluble B-complex vitamin (vitamin B7 or vitamin H) ...

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Biotin 5 g 2-1016-005 USD 159.00
2-1016-005

Biotin is a water-soluble B-complex vitamin (vitamin B7 or vitamin H) ...

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10x Buffer BXT; Strep-Tactin®XT Elution Buffer 25 ml 2-1042-025 USD 62.00  
2-1042-025

*** This article replaces 2-1041-250, 1x Buffer BXT *** ...

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Magnetic Separator for 24 reaction tubes 2-1602-000 USD 225.00  
2-1602-000

Magnetic separator (for 24 reaction tubes, 12x 1.5 ml and 12x 2 ml) for the fast purification of Strep-tag® proteins with MagStrep "type3" XT beads (Strep-Tactin®XT coated magnetic beads) in batch format. Within minutes, the target molecules are bound specifically by Strep-Tactin®XT. Using the magnetic separator, the magnetic beads are separated within seconds from solution.

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MagStrep "type3" XT beads 5% suspension 2 ml 2-4090-002 USD 200.00

 

References

See where to use MagStrep “type 3” XT beads!

  • Protein purification

Essuman K, Summers DW, Sasaki Y, Mao X, Yim AKY, DiAntonio A, Milbrandt J. (2018) TIR Domain Proteins Are an Ancient Family of NAD+ -Consuming Enzymes. Curr Biol. 28(3): 421-430 [Link]

Zhang Y, Ptacin JL, Fischer EC, Aerni HR, Caffaro CE, San Jose K, Feldman AW, Turner CR, Romesberg FE. (2017) A semi-synthetic organism that stores and retrieves increased genetic information. Nature. 551(7682): 644-647. [Link]

  • Pull-down assay

González M, Brito N, González C. (2017) The Botrytis cinerea elicitor protein BcIEB1 interacts with the tobacco PR5-family protein osmotin and protects the fungus against its antifungal activity. New Phytol. 215(1): 397-410. [Link]

  • RNA co-immunoprecipitation

Chen Z, Mashburn-Warren L, Merritt J, Federle MJ, Kreth J. (2017) Interference of a speB 5' untranslated region partial deletion with mRNA degradation in Streptococcus pyogenes. Mol Oral Microbiol. 32(5): 390-403. [Link]