MagStrep "type3" XT beads
Technology
Magnetic Strep-tag® purification at its best! - A superior tool for small scale batch purification with high demands on protein purity.
Magnetic beads are a common tool to isolate proteins or complexes via a pull-down, immunoprecipitation (IP) or co-immunoprecipitation (Co-IP). The advantage of a magnetic beads based pull-down/IP is the simple and reproducible handling of the product. Due to the magnetic core of the beads no centrifugation step is required any more, the beads can be easily separated with a magnetic separator and the risk of disturbing the pellet or loss of beads within the wash step is close to zero.
MagStrep “type3” XT beads are Strep-Tactin®XT coated magnetic beads with the following characteristics:
- high binding capacity (up to 0.85 nmol/μl beads, corresponding to 30 μg of a 30 kDa protein)
- very low non-specific protein binding due to an improved coating
- flexible elution conditions (under denaturing conditions by boiling in SDS gel loading buffer or under native conditions with biotin)
- high affinity for Twin-Strep-tag® – due to new Strep-Tactin®XT
These characteristics lead to very good purification results. MagStrep “type3” XT magnetic beads are therefore a convenient tool for small-scale purifications with high demands on binding specificity as required in pull-down assays and high-throughput screenings.
| Product | Contents | Cat.no | Price | Order | |||
|---|---|---|---|---|---|---|---|
| 10x Buffer W; Strep-Tactin®/Strep-Tactin®XT Wash Buffer | 100 ml | 2-1003-100 | USD 44.00 | ||||
 Strep-Tactin®/Strep-Tactin®XT Wash Buffer is used for the removal of unbound molecules from immobilized Strep-Tactin® and Strep-Tactin®XT during Strep-tag® protein purification. |
|||||||
| Biotin | 2 g | 2-1016-002 | USD 76.00 | ||||
 Biotin is a water-soluble B-complex vitamin (vitamin B7 or vitamin H) ... |
|||||||
| Biotin | 5 g | 2-1016-005 | USD 164.00 | ||||
 Biotin is a water-soluble B-complex vitamin (vitamin B7 or vitamin H) ... |
|||||||
| 10x Buffer BXT; Strep-Tactin®XT Elution Buffer | 25 ml | 2-1042-025 | USD 64.00 | ||||
 *** This article replaces 2-1041-250, 1x Buffer BXT *** ... |
|||||||
| Magnetic Separator | for 24 reaction tubes | 2-1602-000 | USD 232.00 | ||||
 Magnetic separator (for 24 reaction tubes, 12x 1.5 ml and 12x 2 ml) for the fast purification of Strep-tag® proteins with MagStrep "type3" XT beads (Strep-Tactin®XT coated magnetic beads) in batch format. Within minutes, the target molecules are bound specifically by Strep-Tactin®XT. Using the magnetic separator, the magnetic beads are separated within seconds from solution. |
|||||||
| MagStrep "type3" XT beads 5% suspension | 2 ml | 2-4090-002 | USD 206.00 | ||||
| MagStrep "type3" XT beads 5% suspension | 10 ml | 2-4090-010 | USD 715.00 | ||||
References
See where to use MagStrep “type 3” XT beads!
- Protein purification
Essuman K, Summers DW, Sasaki Y, Mao X, Yim AKY, DiAntonio A, Milbrandt J. (2018) TIR Domain Proteins Are an Ancient Family of NAD+ -Consuming Enzymes. Curr Biol. 28(3): 421-430 [Link]
Zhang Y, Ptacin JL, Fischer EC, Aerni HR, Caffaro CE, San Jose K, Feldman AW, Turner CR, Romesberg FE. (2017) A semi-synthetic organism that stores and retrieves increased genetic information. Nature. 551(7682): 644-647. [Link]
- Pull-down assay
González M, Brito N, González C. (2017) The Botrytis cinerea elicitor protein BcIEB1 interacts with the tobacco PR5-family protein osmotin and protects the fungus against its antifungal activity. New Phytol. 215(1): 397-410. [Link]
Jeong, S. J., Park, S., Nguyen, L. T., Hwang, J., Lee, E. Y., Giong, H. K., ... & Kim, H. K. (2019). A threonyl-tRNA synthetase-mediated translation initiation machinery. Nature Communications, 10(1), 1357.[Link]
Maidorn, M., Olichon, A., Rizzoli, S. O., & Opazo, F. (2019). Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons. In MAbs (Vol. 11, No. 2, pp. 305-321). Taylor & Francis. [Link]
- RNA co-immunoprecipitation
Chen Z, Mashburn-Warren L, Merritt J, Federle MJ, Kreth J. (2017) Interference of a speB 5' untranslated region partial deletion with mRNA degradation in Streptococcus pyogenes. Mol Oral Microbiol. 32(5): 390-403. [Link]
