2-1505-010

Strep-Tactin® MacroPrep® resin

50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

€373.00*

Content
Strep-Tactin® MacroPrep® consists of polymethacrylate, a synthetic material coupled with the streptavidin variant Strep-Tactin® and is applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. In contrast to agarose resins, plant cell components such as chlorophyll are not unspecifically bound by this resin, which makes Strep-Tactin® MacroPrep® the favorable alternative.
The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.

Specifications

Bead Size: 50 µm
Biotin Binding Capacity: > 300 nmol/ml resin
Exclusion Limit: 1 x 10^6
Form: 50% suspension
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-Tag®; Strep-tag®II; cross reactivity: biotin
Strep-tag®II Binding Capacity: 3 mg/ml
Support: Polymethacrylate

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

BioLock
Biotin blocking solution for lysates and culture supernatants
Avidin
Efficient blocking of biotin and biotinlyated proteins
10x Buffer W
Strep-Tactin® and Strep-Tactin®XT wash buffer
Desthiobiotin
Desthiobiotin powder for preparation of Strep-Tactin® elution buffer
10x Buffer E
Strep-Tactin® elution buffer with desthiobiotin
10x Buffer R
Strep-Tactin® regeneration buffer with HABA
Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set

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Strep-Tactin® Superflow® high capacity resin
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