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2-1002-001

Strep-Tactin® Buffer Set

Strep-Tactin® purification buffer set

€119.00*

Content
Strep-Tactin® Buffer Set contains all buffers needed for the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins and subsequent resin regeneration.
10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin® and Strep-Tactin®XT resins. During protein purification, it removes unbound molecules from the affinity chromatography matrix. 10x Buffer E is a 10x concentrated Strep-Tactin® elution buffer containing desthiobiotin. Desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® proteins from the modified biotin-binding pocket of Strep-Tactin®. As a result, a mild and highly specific competitive elution occurs. Please note that 10x Buffer E is not applicable for elution from Strep-Tactin®XT resins. 10x Buffer R is a 10x concentrated Strep-Tactin® regeneration buffer containing HABA (2-[4'-hydroxy-benzeneazo]benzoic acid). HABA displaces desthiobiotin from the modified biotin-binding pocket of Strep-Tactin® after elution. Upon binding of HABA to Strep-Tactin®, the yellow buffer turns red, indicating regeneration and column activity. 

Specifications

Concentration: 10x concentrated
Form: Solution
Kit Content: 100 ml 10x Buffer W, 25 ml 10x Buffer E, 100 ml 10x Buffer R
Possible Application: Protein purification with Strep-Tactin® resins

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Content: 1 ml
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Related Products

Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer R (10x)
Strep-Tactin® regeneration buffer with HABA
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer