Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® 50 % suspension can be used for the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. The resin is particularly suited for the purification of proteins with multiple tags, such as multimers, that cannot be eluted from Strep-Tactin®XT resins because of the increased affinity. It can be used for the purification of large proteins, due to the low percentage of agarose. Strep-Tactin® irreversibly binds biotin, so if free biotin is expected use BioLock. The suspension format allows the packing of gravity flow columns with custom resin volumes. For a 1 ml resin bed volume, use 2 ml 50 % suspension.
Strep-Tactin® Sepharose® is not compatible with HPLC/FPLC due to a low pressure stability.
For information on our other resins, please check the resin specifications.
|Bead Size:||45 - 165 µm|
|Biotin Binding Capacity:||> 300 nmol/ml resin|
|Exclusion Limit:||3 x 10^7|
|Possible Application:||Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins|
|Specificity:||Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin|
|Strep-tag®II Binding Capacity:||3 mg/ml|
|Stability:||6 months after shipping|