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2-1201-025

Strep-Tactin® Sepharose® resin

50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Please note that this product will be discontinued and replaced by Strep-Tactin® 4Flow® high capacity resin. Strep-Tactin® Sepharose® resin will still be available until 31st October 2024 or as long as stock lasts. Check out the features of the new resin here

 Strep-Tactin® Sepharose® 50 % suspension can be used for the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. The resin is particularly suited for the purification of proteins with multiple tags, such as multimers, that cannot be eluted from Strep-Tactin®XT resins because of the increased affinity. It can be used for the purification of large proteins, due to the low percentage of agarose. Strep-Tactin® irreversibly binds biotin, so if free biotin is expected use BioLock. The suspension format allows the packing of gravity flow columns with custom resin volumes. For a 1 ml resin bed volume, use 2 ml 50 % suspension.
Strep-Tactin® Sepharose® is not compatible with HPLC/FPLC due to a low pressure stability.

Specifications

Bead Size: 45 - 165 µm
Biotin Binding Capacity: > 300 nmol/ml resin
Exclusion Limit: 3 x 10^7
Form: 50% suspension
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Strep-tag®II Binding Capacity: 3 mg/ml
Support: 4% agarose

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer R (10x)
Strep-Tactin® regeneration buffer with HABA
BioLock
Biotin blocking solution for lysates and culture supernatants

Related Products

Strep-Tactin® Sepharose® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins